pLaminA-mScarlet3-H (aka mYongHong)

LOCUS       pLaminA-mScarlet3-H (aka mYongHong)              6696 bp DNA     circular SYN 30-DEC-2024
DEFINITION  TSN20241013-0571-00010_761_20241014-BAN4_D04_D04_i20.1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6696)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 23, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6696
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        184..387
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      426..435
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             432..1118
                     /codon_start=1
                     /label=mScarlet3-H
                     /translation="MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LRVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYWKQSFPEGFKWERVMNFEDGGA
                     VSVAQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDVVLKGDIKH
                     ALRLKDGGRYLADFKTTYRAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHS
                     TGGSGGS"
     CDS             1158..3152
                     /codon_start=1
                     /label=LaminA/C
                     /translation="METPSQRRATRSGAQASSTPLSPTRITRLQEKEDLQELNDRLAVY
                     IDRVRSLETENAGLRLRITESEEVVSREVSGIKAAYEAELGDARKTLDSVAKERARLQL
                     ELSKVREEFKELKARNTKKEGDLIAAQARLKDLEALLNSKEAALSTALSEKRTLEGELH
                     DLRGQVAKLEAALGEAKKQLQDEMLRRVDAENRLQTMKEELDFQKNIYSEELRETKRRH
                     ETRLVEIDNGKQREFESRLADALQELRAQHEDQVEQYKKELEKTYSAKLDNARQSAERN
                     SNLVGAAHEELQQSRIRIDSLSAQLSQLQKQLAAKEAKLRDLEDSLARERDTSRRLLAE
                     KEREMAEMRARMQQQLDEYQELLDIKLALDMEIHAYRKLLEGEEERLRLSPSPTSQRSR
                     GRASSHSSQTQGGGSVTKKRKLESTESRSSFSQHARTSGRVAVEEVDEEGKFVRLRNKS
                     NEDQSMGNWQIKRQNGDDPLLTYRFPPKFTLKAGQVVTIWAAGAGATHSPPTDLVWKAQ
                     NTWGCGNSLRTALINSTGEEVAMRKLVRSVTVVEDDEDEDGDDLLHHHHGSHCSSSGDP
                     AEYNLRSRTVLCGTCGQPADKASASGSGAQVGGPISSGSSASSVTVTRSYRSVGGSGGG
                     SFGDNLVTRSYLLGNSSPRTQSPQNCSIM"
     polyA_signal    3303..3424
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3431..3886)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        3913..4017
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4019..4376
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4227..4362
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4411..5205
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    5437..5484
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5813..6401
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     enhancer        join(6576..6696,1..183)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
       61 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      121 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      181 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
      241 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
      301 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
      361 cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg ctagcgctac
      421 cggtcgccac catggatagc accgaggcag tgatcaagga gttcatgcgg ttcaaggtgc
      481 acatggaggg ctccatgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
      541 cctacgaggg cacccagacc gccaagctga gggtgaccaa gggtggcccc ctgcccttct
      601 cctgggacat cctgtcccct cagttcatgt acggctccag ggccttcacg aagcaccccg
      661 ccgacatccc cgactactgg aagcagtcct tccccgaggg cttcaagtgg gagcgcgtga
      721 tgaacttcga ggacggcggc gccgtgtccg tggcccagga cacctccctg gaggacggca
      781 ccctgatcta caaggtgaag ctccgcggca ccaacttccc tcctgacggc cccgtaatgc
      841 agaagaagac aatgggctgg gaagcatcca ccgagcggtt gtaccccgag gacgtcgtgc
      901 tgaagggcga cattaagcat gccctgcgcc tgaaggacgg cggccggtac ctggcggact
      961 tcaagaccac ctacagggcc aagaagcccg tgcagatgcc cggcgccttc aacatcgacc
     1021 gcaagttgga catcacatcc cacaacgagg actacaccgt ggtggaacag tacgaacgct
     1081 ccgtggcccg ccactccacc ggcggctccg gtggctcctc cggccggact cagatctcga
     1141 gctcaagctt cgaattcatg gagaccccgt cccagcggcg cgccacccgc agcggggcgc
     1201 aggccagctc cactccgctg tcgcccaccc gcatcacccg gctgcaggag aaggaggacc
     1261 tgcaggagct caatgatcgc ttggcggtct acatcgaccg tgtgcgctcg ctggaaacgg
     1321 agaacgcagg gctgcgcctt cgcatcaccg agtctgaaga ggtggtcagc cgcgaggtgt
     1381 ccggcatcaa ggccgcctac gaggccgagc tcggggatgc ccgcaagacc cttgactcag
     1441 tagccaagga gcgcgcccgc ctgcagctgg agctgagcaa agtgcgtgag gagtttaagg
     1501 agctgaaagc gcgcaatacc aagaaggagg gtgacctgat agctgctcag gctcggctga
     1561 aggacctgga ggctctgctg aactccaagg aggccgcact gagcactgct ctcagtgaga
     1621 agcgcacgct ggagggcgag ctgcatgatc tgcggggcca ggtggccaag cttgaggcag
     1681 ccctaggtga ggccaagaag caacttcagg atgagatgct gcggcgggtg gatgctgaga
     1741 acaggctgca gaccatgaag gaggaactgg acttccagaa gaacatctac agtgaggagc
     1801 tgcgtgagac caagcgccgt catgagaccc gactggtgga gattgacaat gggaagcagc
     1861 gtgagtttga gagccggctg gcggatgcgc tgcaggaact gcgggcccag catgaggacc
     1921 aggtggagca gtataagaag gagctggaga agacttattc tgccaagctg gacaatgcca
     1981 ggcagtctgc tgagaggaac agcaacctgg tgggggctgc ccacgaggag ctgcagcagt
     2041 cgcgcatccg catcgacagc ctctctgccc agctcagcca gctccagaag cagctggcag
     2101 ccaaggaggc aaagcttcga gacctggagg actcactggc ccgtgagcgg gacaccagcc
     2161 ggcggctgct ggcggaaaag gagcgggaga tggccgagat gcgggcaagg atgcagcagc
     2221 agctggacga gtaccaggag cttctggaca tcaagctggc cctggacatg gagatccacg
     2281 cctaccgcaa gctcttggag ggcgaggagg agaggctacg cctgtccccc agccctacct
     2341 cgcagcgcag ccgtggccgt gcttcctctc actcatccca gacacagggt gggggcagcg
     2401 tcaccaaaaa gcgcaaactg gagtccactg agagccgcag cagcttctca cagcacgcac
     2461 gcactagcgg gcgcgtggcc gtggaggagg tggacgagga gggcaagttt gtccggctgc
     2521 gcaacaagtc caatgaggac cagtccatgg gcaattggca gatcaagcgc cagaatggag
     2581 atgatccctt gctgacttac cggttcccac caaagttcac cctgaaggct gggcaggtgg
     2641 tgacgatctg ggctgcagga gctggggcca cccacagccc ccctaccgac ctggtgtgga
     2701 aggcacagaa cacctggggc tgcgggaaca gcctgcgtac ggctctcatc aactccactg
     2761 gggaagaagt ggccatgcgc aagctggtgc gctcagtgac tgtggttgag gacgacgagg
     2821 atgaggatgg agatgacctg ctccatcacc accacggctc ccactgcagc agctcggggg
     2881 accccgctga gtacaacctg cgctcgcgca ccgtgctgtg cgggacctgc gggcagcctg
     2941 ccgacaaggc atctgccagc ggctcaggag cccaggtggg cggacccatc tcctctggct
     3001 cttctgcctc cagtgtcacg gtcactcgca gctaccgcag tgtggggggc agtgggggtg
     3061 gcagcttcgg ggacaatctg gtcacccgct cctacctcct gggcaactcc agcccccgaa
     3121 cccagagccc ccagaactgc agcatcatgt aagtcgacgg taccgcgggc ccgggatcca
     3181 ccggatctag ataactgatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
     3241 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
     3301 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     3361 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     3421 cttaacgcgt aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
     3481 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
     3541 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
     3601 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
     3661 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
     3721 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
     3781 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
     3841 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt
     3901 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     3961 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtcct
     4021 gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     4081 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     4141 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     4201 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     4261 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     4321 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaagatc
     4381 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
     4441 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     4501 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     4561 accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg
     4621 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     4681 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
     4741 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     4801 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     4861 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     4921 ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
     4981 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     5041 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     5101 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     5161 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
     5221 tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg
     5281 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
     5341 agcgcgggga tctcatgctg gagttcttcg cccaccctag ggggaggcta actgaaacac
     5401 ggaaggagac aataccggaa ggaacccgcg ctatgacggc aataaaaaga cagaataaaa
     5461 cgcacggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg
     5521 tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac
     5581 cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc
     5641 ctgccatagc ctcaggttac tcatatatac tttagattga tttaaaactt catttttaat
     5701 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     5761 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     5821 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     5881 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     5941 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     6001 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     6061 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     6121 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     6181 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     6241 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     6301 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     6361 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     6421 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     6481 ctgattctgt ggataaccgt attaccgcca tgcattagtt attaatagta atcaattacg
     6541 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
     6601 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     6661 atagtaacgc caatagggac tttccattga cgtcaa
//